FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734009
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30150.4107579062382069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.21443878753530268No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT8670.11811844268939482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4350.053.649151
AACGGAT200.007111273252.19821544
GGTATCA17800.046.4665341
TGGTATC5300.043.3822982
GTATCAA50400.040.924851
TATCAAC56650.036.4072722
ATCAACG60500.034.317343
CGTATGC2250.033.93403641
TCGTATG2050.033.80287640
TCAACGC61200.033.7005274
CAACGCA61850.033.3463555
CTCGTAT1800.032.69381739
AACGCAG63500.032.533926
TGTACGA450.00447728930.86614442
CGCAGAG68450.030.2649778
ACGAGAC2500.030.197222
TCTCGTA1850.029.90405838
TATACAC2650.029.784863
ACGCAGA69850.029.6603987
GCAGAGT70800.029.2119759
Produced by FastQC (version 0.11.5)