FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179237
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC91300.4189539733402104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79620.36535723282965554No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA57690.2647256815114648No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53860.24715072293651402No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52910.24279139900800142No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49410.22673073190295503No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48260.2214536555684398No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG46900.21521293920762175No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT44920.2061271903881955No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44150.20259384362508528No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA44140.20254795600478515No Hit
GGTCTAGTCAGAGCCTCCTGTATAGTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC43460.19942759782437613No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG43090.19772975587327124No Hit
CTACTATACAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC42160.1934622071853589No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT42150.19341631956505878No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCGGGCACAGATTTTACATTGAC41520.19052539948615044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40380.1852942107719353No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTGTCAATGTAAAAT39130.17955825823441873No Hit
CTACAAACTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG38950.17873228106901634No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC36900.1693253189074892No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTGTCAATGTAAAATCTGTGCCCGATCCACTGCCA35960.1650118825992767No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG35460.1627175015842701No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34830.15982658150536175No Hit
GTCTATTACTGTGCGAGAAATCAACCTTATTGTCTTAATGGTGTATGTTC32750.15028195648293416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32520.1492265412160311No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTGATCGGGCC32370.14853822691152913No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31590.14495899252811878No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31480.14445422870481733No Hit
GTCAGGGACCCCGGAGGCCCGATCAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA31300.14362825153941494No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31230.14330703819731402No Hit
GTTCAGTGGCAGTGGATCGGGCACAGATTTTACATTGACAATCAGCAGAG30360.13931481523120248No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG28420.13041261689297676No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28360.13013729117117598No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG28310.1299078530696753No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC28290.12981607782907503No Hit
GGCCAAAAGTGTAAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA28220.12949486448697412No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTTACACTTTTGGCCAGGGGA26890.12339181098705648No Hit
CTCCTGTATAGTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT26640.12224462047955317No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACT26410.12118920521265013No Hit
GTGTAAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTC26360.12095976711114945No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26220.12031734042694761No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26100.119766688983346No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26010.11935370040064482No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGA25960.11912426229914415No Hit
GCAGTGGATCGGGCACAGATTTTACATTGACAATCAGCAGAGTGGAGGCT25840.11857361085554256No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTGTCAATGTAAAATCTGTGCCCGATCCACTGCCA25620.11756408320893964No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT25590.11742642034803924No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25530.11715109462623846No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25450.11678399366383738No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25440.11673810604353725No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25410.11660044318263685No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG25380.11646278032173647No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25300.11609567935933542No Hit
CCTGTATAGTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAG25030.11485671361123181No Hit
GTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT24660.11315887166012692No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC24610.11292943355862624No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACTCTCC24530.11256233259622518No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24520.11251644497592506No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24500.1124246697353248No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24470.1122870068744244No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24430.11210345639322387No Hit
ATTTGGGTTCTGATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCGGGCACAGATTTTACATTGA24200.11104804112632083No Hit
CTCCAGCCCCTTCCCTGGGGCCTGGCGGACCCAGGTCATCCAATAGCTCT24180.11095626588572055No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23990.11008440110001803No Hit
GATTTTACATTGACAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT23930.10980907537821724No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23640.10847833438951339No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG23610.10834067152861299No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAAGAGCTATTGGATGACCTGGGTCCGCCAGGCCCCAGGGAAGGGG23540.10801945818651208No Hit
CCATTAAGACAATAAGGTTGATTTCTCGCACAGTAATAGACGGCCGTGTC23430.10751469436321062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23390.1073311438820101No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23150.1062298409948069No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA22720.1042566733219012No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTGATCGGGCCTCCGGGGTCCCTGA22620.10379779711889986No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22440.1029718199534975No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21910.10053977607759046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6500.054.0855751
TGGTATC7050.049.8661352
GGTATCA23950.046.338821
GTATCAA63450.041.6637461
TATCAAC71950.036.5979842
ATCAACG76100.034.91923
TCAACGC76400.034.7369734
CAACGCA77700.034.2001465
AACGCAG78150.033.9591146
TCGTATG3100.032.37194440
CGCAGAG84050.031.6061618
AGAGTAC84500.031.47861911
CAGAGTA85450.031.12865310
ACGCAGA86400.030.7472087
TCTGGAG26550.029.65932870
CGTATGC3200.029.21302241
ATGGACG16450.028.484470
CTTATAC3350.027.7788071
GAGTACT36300.027.33637612
AGTACTT36450.027.12935413
Produced by FastQC (version 0.11.5)