FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179237
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC78830.3617321108259451No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73220.33598915583757066No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA50770.23297144826377306No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49440.2268683947638554No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA46810.21479995062492055No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46690.21424929918131894No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG45930.21076184003850887No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44830.2057142018054943No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41580.19080072520795122No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT41020.18823101847114382No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT40580.18621196317793798No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38110.17487772096380522No Hit
GGTCTAGTCAGAGCCTCCTGTATAGTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC37340.171344374200695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36420.1671227131330828No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG36350.16680149979098188No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35540.16308460254667115No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTGTCAATGTAAAAT35410.16248806348276942No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCGGGCACAGATTTTACATTGAC35360.16225862538126878No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG35240.16170797393766717No Hit
CTACTATACAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC35180.1614326482158664No Hit
CTACAAACTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG34410.15789930145275619No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTGTCAATGTAAAATCTGTGCCCGATCCACTGCCA34350.15762397573095538No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34100.15647678522345207No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33400.15326465180244278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC33220.15243867463704042No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31820.14601440779502184No Hit
GTCTATTACTGTGCGAGAAATCAACCTTATTGTCTTAATGGTGTATGTTC30220.13867238854700062No Hit
GTTCAGTGGCAGTGGATCGGGCACAGATTTTACATTGACAATCAGCAGAG29320.1345425027199887No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28900.13261522266738313No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28240.12958663972757437No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27560.12646628154716538No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27300.1252732034193619No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG27220.12490610245696086No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27020.12398835005095821No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTGATCGGGCC26770.12284115954345488No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT26760.12279527192315476No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26590.12201518237805249No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC26350.12091387949084932No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG25950.11907837467884402No Hit
GTCAGGGACCCCGGAGGCCCGATCAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA25780.11829828513374176No Hit
GTGTAAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTC25340.11627922984053594No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACT25280.11600390411873514No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25190.11559091553603396No Hit
GGCCAAAAGTGTAAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA25080.11508615171273248No Hit
CTCCTGTATAGTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT25040.11490260123153195No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24960.1145355002691309No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACTCTCC24810.11384718596462891No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24780.1137095231037285No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24760.11361774786312823No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTTACACTTTTGGCCAGGGGA24690.11329653452102732No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24600.11288354593832611No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24490.11237878211502465No Hit
ATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATCAGAACCCAAATAGATCAGGAGCTGTGGAGA24070.11045150206241909No Hit
GCAGTGGATCGGGCACAGATTTTACATTGACAATCAGCAGAGTGGAGGCT23910.10971730013761696No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23840.10939608679551603No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23750.10898309821281485No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAAGAGCTATTGGATGACCTGGGTCCGCCAGGCCCCAGGGAAGGGG23520.1079276829459118No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGA23380.10728525626170994No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22940.10526620096850411No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22700.10416489808130094No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22410.10283415709259709No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22290.1022835056489955No Hit
ATTTGGGTTCTGATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCGGGCACAGATTTTACATTGA22180.10177874182569405No Hit
GTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT22180.10177874182569405No Hit
CCTGTATAGTAGTGGATCCAACTATTTGGATTGGTACCTGCAGAAGCCAG22030.10109042752119204No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTGTCAATGTAAAATCTGTGCCCGATCCACTGCCA22030.10109042752119204No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA21980.10086098941969139No Hit
GATTTTACATTGACAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT21940.10067743893849086No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG21900.10049388845729033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)