FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522690
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22000.040.4527971
GGTATCA9300.039.534141
CCGATAC350.001363102739.3342130
GTGGTAT3150.037.088551
TATCAAC25550.034.8321532
TCGTATG400.002539234534.6532440
CAACGCA26500.033.5834545
TCAACGC26500.033.5834544
ATCAACG26850.033.1456833
AACGCAG27450.032.796726
TGGTATC3600.032.452482
ACGCAGA30100.030.0177147
CGCAGAG29800.029.974058
AGAGTAC30500.029.06084411
GCAGAGT30950.028.8603179
CAGAGTA31350.028.27291110
GCGTGTT500.00737165827.87050647
CGACCGT500.00775276327.58417533
CGACGAA500.00789179227.48389814
GAGTACG19200.026.30294812
Produced by FastQC (version 0.11.5)