FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522690
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13570.2596185119286767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27150.041.8703541
ATACGAA350.00136459739.3254332
GGTATCA10600.038.8797871
GTGGTAT4000.036.9195981
TATCAAC31650.036.0188332
TTTCGGC400.002663417334.31750525
CAACGCA33500.034.1888735
TCAACGC33500.034.086514
ATCAACG33550.034.0520023
TGGTATC4150.033.9235272
AACGCAG34050.033.734116
ACGCAGA36300.031.6431547
CGCAGAG36500.031.4697678
AGAGTAC37350.030.75358811
CAGAGTA38100.030.23819710
GCAGAGT38050.030.187829
GAGTACG22850.027.91054512
AGTACGG23000.027.87759613
GTACGGG25150.025.22175614
TACGGGG8750.025.07919115
Produced by FastQC (version 0.11.5)