FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301956
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12750.040.7335661
ACGGGGC1300.037.02768316
TATCAAC14500.035.1058432
AGGACGT501.8920058E-434.3942386
CAGGACG400.002632655234.3942385
GTGGTAT2100.034.3942341
ATCAACG15100.033.4831283
TCAACGC15350.032.93784
GGTATCA5850.032.9243971
CAACGCA15850.031.8987565
TCGTATG450.004537195430.77657340
AACGCAG16450.030.7352736
ACGCAGA17900.028.236197
CGCAGAG17950.028.1575378
TGGTATC2600.027.779962
CAGAGTA18750.027.322910
AGAGTAC18750.027.13952611
GCAGAGT19050.026.5316459
ATAGCGG808.141581E-526.4844463
ATAGCAC658.7985786E-426.4571043
Produced by FastQC (version 0.11.5)