FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842458
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.1433411236511226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27850.040.5711021
GTATCAA64900.039.2254451
TATCAAC72150.035.188382
TCAACGC77850.032.7889674
ATCAACG77650.032.78473
GTGGTAT11200.032.2953071
CAACGCA80700.031.6736815
AACGCAG82300.031.057916
TGGTATC11800.029.777372
ACGCAGA89800.028.4570377
CGCAGAG92100.027.7837778
CAGAGTA96950.026.46491810
GCAGAGT97900.026.2432869
AGAGTAC98250.026.07969311
AGTACGG57300.024.52266313
GAGTACG58150.024.22343312
TACGGGT5950.021.9976315
AGTACTT54650.021.11138513
GTACGGG67700.020.65433714
TAACGGC1055.9624633E-419.74478736
Produced by FastQC (version 0.11.5)