FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842458
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34220.1857301496153508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.041.869651
TGGTATC10600.039.9553572
GTATCAA68750.038.4868431
GTGGTAT11650.037.262191
TATCAAC78100.033.8599242
ATCAACG82950.031.988183
TCAACGC83250.031.8056414
CAACGCA84850.031.1155415
AACGCAG86800.030.5336726
ACGCAGA94650.028.0376197
CGCAGAG95600.027.7230428
CAGAGTA98900.026.69373510
AGAGTAC100800.026.39519311
GCAGAGT100150.026.3605639
AGTACTT49200.023.68723513
AGTACGG64900.022.77736713
GAGTACG66900.022.29955912
GAGTACT53850.021.76713812
TACTTTT55950.020.9598115
ACTTTTT58600.020.30705516
Produced by FastQC (version 0.11.5)