FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014018
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.13747290482023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.041.6889841
GTATCAA42150.039.3702351
TATCAAC48550.034.1097152
ACATTCG450.00394652931.67343765
ATCAACG54200.030.6172583
TCAACGC54100.030.6104744
CAACGCA54750.030.4975575
AACGCAG56250.029.7452436
CTCGTAT1750.029.64117239
GTGGTAT6750.028.9528471
TCTCGTA2150.027.30808438
CGCAGAG61900.026.9655088
ACGCAGA62200.026.8918767
TGGTATC7200.026.1908992
AGAGTAC63200.026.13967711
CAGAGTA64750.025.56687410
TATCTCG2300.025.49545136
GCAGAGT66050.025.2712339
TCGTATG2350.023.57760440
AGCGTCA2053.6379788E-1223.4150123
Produced by FastQC (version 0.11.5)