FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014018
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17970.1772157890688331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50350.039.931211
GGTATCA20600.039.306031
GTGGTAT7650.036.32771
TATCAAC57150.034.859142
TCAACGC61800.032.3579334
TGGTATC8700.032.3185162
ATCAACG62050.032.251433
CAACGCA62100.032.1857345
AACGCAG64200.031.238016
GTGCGAA850.005740140629.33567870
CGCAGAG69250.029.0094248
ACGCAGA69550.028.8350837
AGAGTAC71550.027.74071911
GCAGAGT73100.027.3879349
CAGAGTA74000.026.77735110
ACCGAGT907.35094E-626.6180538
AGTACGG41900.023.60735913
GAGTACG43150.023.55572312
GAGTACT40850.023.54154812
TACGGGG16650.023.44251315
Produced by FastQC (version 0.11.5)