FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161125
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATGATCACGA30870.26586284853052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30020.2585423619334697No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25380.2185811174507482No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT24850.21401657874905805No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATGATCACGACTTTTATCCCACAC23920.20600710517816773No Hit
CCTATGTGCTGACTCAGACACCCTCGGTGTCAGTGGCCCCAGGAAAGACG23750.20454300785875765No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23400.20152868984820754No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC21760.18740445688448704No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21300.1834427817849069No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19790.1704381526536764No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19490.1678544515017763No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18270.1573474001507159No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18230.1570029066637959No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17870.15390246528151577No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17190.14804607600387557No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16930.14580686833889547No Hit
GGATTACCTGTGGGGGAGACAACATTGGAACTAAAAGTGTGCACTGGTTCCAGCAGAGGCCAGGCCAGGCCC16880.14537625148024544No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG16690.14373990741737538No Hit
CCCCTGTGCTGGTCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCT16660.14348153730218538No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16490.14201743998277533No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16340.14072558940682528No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG16170.13926149208741523No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGGTATCATCATAGA16050.13822801162665518No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15900.13693616105070514No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATACCGACCGGCCC15790.1359888039616751No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15260.13142426525998493No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT15050.12961567445365488No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATACCGACCGGCCCTCAGG15000.12918505759500484No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGACAC14620.1259123694692647No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14600.12574012272580473No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT14550.1253095058671547No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14540.1252233824954247No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC14520.1250511357519647No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14420.12418990203466465No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14100.12143395413930455No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13950.1201421035633545No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13790.11876412961567444No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13790.11876412961567444No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13780.11867800624394446No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13650.1175584024114544No Hit
TGATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC13310.1146302077726343No Hit
GTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCCTCTGCTGGAA12860.11075465604478416No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGACACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCTGGATTAC12470.10739584454731402No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGACACCCTCGGTGTCA12280.10575950048444396No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12200.10507051351060395No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11950.10291742921735385No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11780.10145333189794381No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG11740.1011088384110238No Hit
CCACATGATCACGACTTTTATCCCACACCTGACAGTAATAGTCGGCCTCA11680.10059209818064378No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGA11650.10033372806545375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5000.050.934171
TGGTATC5500.045.6426052
GTATCAA44150.041.7812651
TATCAAC50100.036.6533622
ATCAACG53650.034.141843
TCAACGC54000.033.839414
CAACGCA55450.033.0093575
AACGCAG56550.032.6708956
CGCAGAG59750.031.0361068
AGAGTAC61000.030.45641511
CAGAGTA61700.030.05522310
GCAGAGT61950.030.0448029
GGGCAGT4100.026.860471
ACGCAGA70000.026.4915337
AGTAATA16450.026.1259970
GAGTACT25300.025.9242112
GAGTACG46900.025.69963512
GGTATCA28100.025.5969051
AGTACGG46700.025.51776913
GGGTATC951.07107935E-525.3584061
Produced by FastQC (version 0.11.5)