FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953057
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.1054501462137102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC200.007499497451.5024267
GGTATCA18350.040.9860081
GTATCAA42250.038.203111
GTGGTAT6200.037.1116871
TCGTATG1600.034.65860740
TATCAAC48300.033.4178352
CGTATGC1700.032.6388841
TGGTATC7150.032.180762
TCAACGC50450.031.9256134
ATCAACG50750.031.8045583
CTCGTAT1650.031.47282639
CAACGCA51400.031.4023635
ACCGTAC450.004732005830.5199558
AACGCAG53750.030.1572066
TCTCGTA1650.029.34824438
ACGCAGA58100.027.8934547
CGCAGAG58200.027.6685418
AGAGTAC59050.027.2121211
TATGCCG2050.027.11667843
CAGAGTA60100.026.8509610
Produced by FastQC (version 0.11.5)