FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953057
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19560.20523431442190762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6400.045.567011
GGTATCA21150.040.716921
GTATCAA48650.038.011721
TGGTATC7700.037.844032
TATCAAC54000.034.3457222
ATCAACG57150.032.4390143
TCAACGC57100.032.1437154
CAACGCA57650.032.003495
AACGCAG59600.031.3584256
CGCAGAG64000.029.2560218
ACGCAGA64150.029.1342497
AGAGTAC66500.028.1561711
CAGAGTA67550.027.61715910
GCAGAGT68550.027.1144169
ATACGAC1405.0386006E-1026.93321226
GAGTACG40050.025.89691212
AGTACGG39500.025.6549313
ACGGGCT5350.024.97110716
CGGGATA1052.4072995E-522.83922417
GTACGGG44400.022.67299814
Produced by FastQC (version 0.11.5)