FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586322
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.13047438097154807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.037.435141
GTATCAA27150.037.3363571
GTGGTAT4250.037.1919251
CGTATGG400.002538903834.6548640
TATCAAC30950.032.7522472
ATCAACG32100.031.9000233
TCAACGC32050.031.8425754
CTCGTAT901.9330218E-730.77254539
CAACGCA33250.030.6933695
CGAGATA450.00471031130.54669828
TGGTATC5400.029.9077572
AACGCAG34450.029.5244796
TCGTATG953.076202E-729.18304340
TCTCGTA953.1346462E-729.1203938
CGGTACA605.514324E-428.66443831
ACGCAGA37000.027.3827937
CGCAGAG37600.026.8522018
GCAGAGT39350.025.8325569
CAGAGTA39150.025.78908510
AGAGTAC39550.025.52826311
Produced by FastQC (version 0.11.5)