FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997639
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.14323818535562463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.040.540371
GTATCAA44650.037.7753141
TATCAAC50900.033.1385542
ATCAACG53050.031.989793
GTGGTAT7000.031.8979931
TCAACGC53250.031.8051244
CAACGCA54700.031.0876355
TGGTATC7100.030.9664542
AACGCAG56500.030.2188366
CGCGTAT605.332395E-428.83168439
TCGCGTA605.3434365E-428.82149538
TCGTATG1452.1827873E-1128.66324240
ACGCAGA60700.028.229787
CGCAGAG60650.028.1398148
AGAGTAC62400.027.45932211
CAGAGTA63650.026.86611210
GCGGTAC1057.2048897E-726.47940844
GCGTTAC858.39688E-526.37329368
TAACGGC1057.617E-726.31123236
GCAGAGT65250.026.2073259
Produced by FastQC (version 0.11.5)