FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997639
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24800.24858691370325336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47150.040.8404461
GTGGTAT7300.038.5567441
GGTATCA18750.037.894691
TATCAAC53600.035.6446572
ATCAACG56400.033.920563
TCAACGC56500.033.782894
TGGTATC8250.033.677132
CAACGCA58300.032.9013375
AACGCAG60450.031.7311536
ACGCAGA64250.029.8544467
CGCAGAG64700.029.6997418
AGAGTAC66400.028.73157511
CAGAGTA67250.028.47171610
GCAGAGT68250.028.154929
GTACGTA500.00792885727.4599231
GAGTACG38900.025.53538912
AGTACGG38700.025.49161713
TTACGAT700.001379893724.4993326
GAGTACT39800.023.15065812
AGTACTT39850.022.95085313
Produced by FastQC (version 0.11.5)