FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064898
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT59400.5577999019624416No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55150.5178899763169806No Hit
GTTTTATACAACTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCAGAA46790.439384804929674No Hit
ACTTAGGAGGCTGTCCTGCTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGTTGTATAAAACACT39180.36792256159744874No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38350.36012838788315876No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36880.34632424889519936No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35400.3324262041998388No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32270.3030337177832994No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31740.29805671529104194No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30230.2838769534734782No Hit
CTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT29240.2745802884407708No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28390.26659830331167866No Hit
ATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA27920.2621847350638277No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAGCAGCC26950.2530758814459225No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT26570.2495074645646813No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC25870.24293406504660542No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25100.2357033255767219No Hit
GTCTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA24900.2338252114287002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24720.2321349086954807No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23190.21776733546311475No Hit
GTCTATGGTGCGTCCTTCAGTGGTTACTACTGGAGTTGGATCCGCCAGCC22820.21429282428927465No Hit
AAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAA21310.2001130624717109No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTA20970.19692026842007404No Hit
GTAATAGACAGCCGTGTCCGCGGCGGTCACAGACCTCAGCCTCAGGGAGA20640.19382138007583824No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG20640.19382138007583824No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA20380.19137983168341005No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGCTTTCTGCTGGTA20210.18978343465759162No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20180.18950171753538836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20140.189126094705784No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACGGCCAGGGAGTCTGGAGA19770.18565158353194391No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC19280.18105020386929077No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19040.17879646689166476No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAAGAACTT19020.17860865547686255No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18120.17015714181076497No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18050.16949980185895738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17960.16865465049234762No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17950.16856074478494654No Hit
CTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAA17920.1682790276627433No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17320.16264468521867823No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17300.16245687380387605No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17260.1620812509742717No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17160.16114219390026088No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17110.16067266536325545No Hit
GTCTATTACTGTGTGAGAGTCAGATGGGGTATAACTGGAGCGACGCGCTCCTCTTTTTACTCTGACTACTGGGGC16110.151282094623147No Hit
GTCTGAGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG15940.14968569759732855No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15940.14968569759732855No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15790.1482771119863123No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15410.14470869510507112No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15030.14114027822382988No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAG14920.14010731544241797No Hit
GTCCTGCTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGTTG14730.13832310700179737No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14610.13719623851298435No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14490.13606937002417133No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14410.13531812436496266No Hit
CTTCTTAGCTTGGTACCAGCAGAAAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATC14350.13475469012055613No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14340.13466078441315504No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14340.13466078441315504No Hit
CCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14010.1315618960689193No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13880.1303411218727052No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGG13560.12733613923587048No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT13550.1272422335284694No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAAT13210.12404943947683252No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13210.12404943947683252No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13170.1236738166472282No Hit
GACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAG13110.12311038240282168No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAA12910.12123226825479999No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12890.12104445683999783No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12790.12010539976598697No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12780.1200114940585859No Hit
GCAGAAAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATT12540.11775775708095988No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12500.11738213425135553No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12430.11672479429954793No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12170.11428324590711973No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12120.11381371737011434No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12110.11371981166271322No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11840.11118435756288395No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11710.10996358336666986No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11700.10986967765926876No Hit
TTATACTACTCCTCGTACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT11640.10930624341486228No Hit
GTACCAGCAGAAAGCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGA11570.10864890346305468No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGATGA11490.107897657803846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.10686469502243408No Hit
CTCCAGACTCCCTGGCCGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC11340.10648907219282973No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11290.10601954365582432No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11280.10592563794842322No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG11020.10348408955599504No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10960.10292065531158853No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10860.10198159823757767No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10750.10094863545616575No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10730.10076082404136359No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10720.10066691833396249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3850.046.462551
GGTATCA12200.045.67891
TGGTATC4000.045.580212
GTATCAA30000.039.216181
ACGAGAC1550.037.73452422
CCATTAC557.542365E-637.5274473
TACTGAT8550.036.67954670
TATCAAC33900.034.806062
CGTATGC1400.034.58699441
ATCAACG36400.032.510043
CTTATAC1800.032.489041
TATGCCG1600.032.4482943
TCAACGC36750.032.1068154
CTCGTAT1305.456968E-1231.88880539
CAACGCA37550.031.331175
TCGTATG1550.031.22217240
TTAGATC450.004401632630.97512856
AACGCAG38250.030.8477236
CGAGACG1700.030.36449423
GCCGTCT1501.8189894E-1230.0534646
Produced by FastQC (version 0.11.5)