FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451449
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.10654581137625734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGA200.006548734453.29380465
ACGAGAC851.8189894E-1244.39471422
TCGTATG803.274181E-1143.30829640
CGTATGC901.8189894E-1242.3838341
GACCGGA903.926234E-641.25193470
GGTATCA8250.041.1751331
GTATCAA19500.039.7674371
GTGGTAT2800.039.2144131
CTCGTAT852.8740033E-936.63957639
TATCAAC23550.033.0741542
ATCTCGT1056.348273E-1032.88640237
ATCAACG23900.032.4462363
TCAACGC24150.032.110354
CGATTAA652.238365E-532.01016641
CAACGCA24250.031.977945
ACCTAGA553.3595067E-431.1932811
AACGCAG25200.030.9085836
GACAACG450.00446813730.87647243
TATGCCG1251.1459633E-1030.56770943
TGGTATC3750.029.2800942
Produced by FastQC (version 0.11.5)