FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451449
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10320.22859725018772883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT150.00204875871.46335665
GTATCAA21600.041.0151861
GTGGTAT3650.037.6310121
TATCAAC24100.036.8824962
TCAACGC24650.035.8486824
ATCAACG24800.035.7937623
GGTATCA8800.035.508921
CAACGCA25200.035.0507355
AACGCAG26050.034.1698886
TGGTATC4300.032.7229922
ACGCAGA27700.032.13457
CGCAGAG27850.031.7155678
AGAGTAC28350.031.1562111
CAGAGTA28950.030.51048310
GCAGAGT29100.030.3532149
AACGCTA755.164263E-528.32014761
GAGTACG17700.027.27242712
AGTACGG17600.026.84382213
CGCTAAG807.828699E-526.6416863
GCGGTAC959.923035E-625.60341844
Produced by FastQC (version 0.11.5)