FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437232
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27450.6278131518278626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.14248728363889196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG300.00433788359.13234770
GGTATCA9000.043.222331
GTATCAA24650.042.6363141
GTGGTAT2350.040.6570631
TATCAAC28800.036.577492
ATCAACG29900.035.1097763
TCAACGC30300.034.734914
CAACGCA31200.033.9473955
AACGCAG32150.032.8383566
TGGTATC2900.031.7404962
CGCAGAG34500.030.5028278
ACGCAGA35000.030.1643777
AATACGA704.1450323E-529.24819625
AGAGTAC35900.028.83897411
GCAGAGT36550.028.4192859
CAGAGTA36700.028.3031310
TACGACT500.00812369627.32022927
AGTACGT500.00823337827.24524513
GAGTACT24350.026.43403812
ATACGAC659.2154235E-426.25138926
Produced by FastQC (version 0.11.5)