FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209258
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCAACGGGGACTTTTGGCCAGGGGA31640.261648051945904No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30650.25346121340524524No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAA23550.19474752286112643No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22260.18407982415663157No Hit
CAGTAATACACTGCAAAATCTTCCGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21130.1747352508728493No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19710.16299251276402554No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19690.16282712208643646No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCGGAAGATT19350.16001548056742235No Hit
GTCTCTGGTGGCTCCATCAGTAAATTCTACTGGAGCTGGATCCGGCAGCC18800.155467236933723No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18160.15017473525087285No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18110.1497612585569002No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17880.1478592657646259No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17330.14331102213092656No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16860.13942434120758349No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCAACGGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA16710.1381839111256655No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16570.13702617638254203No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16430.13586844163941855No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAACTTCTTGGCCTGGTACC16230.13421453486352788No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG15980.13214715139366454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.1303278539401848No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15240.12602769632286906No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14910.12329875014264946No Hit
GTATGGTAGCTCAACGGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT14790.12230640607711506No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14750.12197562472193693No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14530.12015632726845718No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14420.11924667854171732No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13390.11072905864588037No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC13060.10800011246566077No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12310.10179796205607075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4300.044.850191
GTATCAA40950.042.8063851
TGGTATC4300.041.6466032
GGTATCA14800.041.1866261
TATCAAC45350.038.6531752
ATCAACG49050.035.8778653
TCAACGC49600.035.5494584
CAACGCA50200.035.261775
AACGCAG51200.034.236756
TATACAC3750.033.9793553
CTTATAC3500.033.454581
CGTATGC3400.032.58796741
TCGTATG3450.032.09565740
CGCAGAG55250.031.9035248
AGAGTAC55200.031.74532111
ACGCAGA57300.030.6444977
CAGAGTA58000.030.39085810
GCAGAGT58100.030.3385529
AGTACGG37700.028.85670513
GTCGTAT605.3531356E-428.8131939
Produced by FastQC (version 0.11.5)