FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209258
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34810.28786247434377116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28800.2381625757282565No Hit
GTATTACTGTCAGCAGTATGGTAGCTCAACGGGGACTTTTGGCCAGGGGA27580.2280737443953234No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24160.19979193852759294No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAA21600.17862193179619237No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20190.16696188902616316No Hit
CAGTAATACACTGCAAAATCTTCCGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19230.15902313650188793No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18960.15679036235443553No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18460.15265559541470886No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCGGAAGATT17190.1421532873878031No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17100.1414090293386523No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16760.13859738781963815No Hit
GTCTCTGGTGGCTCCATCAGTAAATTCTACTGGAGCTGGATCCGGCAGCC15620.12917011919706134No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCAACGGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA15400.12735082174358162No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14790.12230640607711506No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAACTTCTTGGCCTGGTACC14720.12172753870555333No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14340.11858511583136104No Hit
GTATGGTAGCTCAACGGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT14120.11676581837788132No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13600.11246566076056556No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13440.11114253533985304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13430.1110598400010585No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13270.10973671458034598No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG12930.10692507306133182No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12720.10518847094664661No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC12450.10295569679919421No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12410.10262491544401608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)