FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746174
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22160.29698166915491564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.12557392779700177No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC8320.11150214293180946No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT8160.10935787095235158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA200.00689731952.6030448
GCGTATC200.006935041452.53054447
TGGTATC6600.048.594972
GTGGTAT6900.046.5039981
GGTATCA21300.043.59141
GTATCAA57450.040.7016561
ACGCATT1205.2695214E-737.30452370
TATCAAC67550.034.5997242
ATCAACG69300.033.7056243
TCAACGC69850.033.4576154
CAACGCA71150.032.8831635
AACGCAG72200.032.4521186
ACGCAGA76900.030.2915527
GTACGTT450.004914361530.28404614
CGCAGAG77050.030.188388
GCAGAGT78600.029.5064079
AGAGTAC79600.029.34964411
CAGAGTA80450.028.99721310
TATTTCG900.006696771428.42249570
AACGCAT1054.4932185E-628.35470269
Produced by FastQC (version 0.11.5)