FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891952
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33320.37356270292571797No Hit
CTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG28870.3236721258543061No Hit
GGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT27550.30887312321739285No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23140.25943100077134196No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG22820.2558433637684539No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22240.24934077170071933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19460.2181731752381294No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18970.2126796060774571No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18890.21178269682673506No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGT17250.19339605718693384No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16590.18599655586847724No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16430.18420273736703321No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15480.1735519400147093No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15440.1731034853893483No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15290.17142178054424453No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA15200.17041275763718225No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA15140.16974007569914076No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15080.16906739376109925No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14790.16581609772723196No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14240.15964984662851814No Hit
GGCCAAAAGTGTACGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA13170.1476536854001112No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG12970.14541141227330617No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTCGGGTTCTAATCGGGCC12960.14529929861696594No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12440.13946938848727286No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12210.1368907743914471No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12140.13610597879706532No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12100.13565752417170432No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11970.13420004663928103No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG11590.12993972769835146No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11550.1294912730729905No Hit
TTATTACTGCATGCAAGCTCTACAAACCCCGTACACTTTTGGCCAGGGGA11460.1284822501659282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11390.12769745457154646No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACC11360.1273611136025257No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11200.12556729510108167No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11040.12377347659963764No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10970.12298868100525588No Hit
CTAATGGAAGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAG10880.12197965809819361No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10390.1164860889375213No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10000.1121136563402515No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9980.111889429027571No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC9930.11132886074586973No Hit
GTTTATTACTGCATGCAAGCTCTACAAACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA9830.11020772418246721No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA9800.10987138321344647No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9770.1095350422444257No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9390.10527472330349615No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9260.10381724577107289No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAGTCTCC9160.10269610920767037No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9110.1021355409259691No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCGAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA9070.1016870863006081No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8950.10034172242452508No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8930.10011749511184458No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8920.10000538145550433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5150.051.4991761
TGGTATC5350.048.891712
GTATCAA30350.039.6080551
GGTATCA12850.036.990721
TATCAAC34550.034.7658422
CGCTCGC400.002634757434.39595816
ATCAACG36800.032.440423
TCAACGC37250.032.0359424
CAACGCA37750.031.6938385
AACGCAG38700.030.8252566
CGCAGAG41350.028.93298
ACGCAGA41400.028.8149157
AGAGTAC42450.028.5055111
CAGAGTA43050.028.10979710
GCAGAGT43600.027.5975029
CGGGATT1359.727955E-925.47848517
TTAGGTC700.001358619724.5644074
AGTACGG30050.024.48534213
GAGTACG30300.024.28059812
AGTACTT19100.024.12172313
Produced by FastQC (version 0.11.5)