FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065944
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19260.18068491402925482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.041.2690731
GTATCAA49200.039.431171
GTGGTAT8300.035.163911
TATCAAC55850.034.469152
TGGTATC8700.033.5266842
ATCAACG59400.032.325533
TCAACGC59400.032.1874124
CAACGCA60850.031.4130465
AACGCAG62900.030.3333636
ACGCAGA67500.028.113967
CGCAGAG69200.027.5718028
AGAGTAC70200.027.51931811
CAGAGTA71350.026.98102210
GCAGAGT71600.026.7432889
AGTACGG46050.024.54826213
GAGTACG46000.024.42371412
TACGGGT8950.023.73819515
GTACGGG50950.021.9887814
ACGGGTT3900.021.96823716
GAGTACT37200.021.54609512
Produced by FastQC (version 0.11.5)