FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152752
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63260.548773717156856No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45500.3947076214137994No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42390.3677287048732078No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39510.342745013671631No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38810.33667258872680333No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA38130.3307736616375422No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG37330.32383374741488197No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG36650.31793482032562076No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35730.30995391896956154No Hit
CTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT35190.30526947686926587No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC34980.3034477493858176No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34300.2975488222965564No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT33770.29295112912404403No Hit
GTCCCTGACAGGTTCAGTGGCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGAT32990.28618471275695034No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32020.2777700667619748No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC30440.2640637361722209No Hit
GTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTT29500.2559093369605952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29330.25443460518827987No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29160.2529598734159646No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28710.2490561716657182No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCACT27450.2381258067650284No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG26920.233528113592516No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCC26730.2318798839646342No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26230.22754243757547157No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAAACT23550.20429372492955988No Hit
CTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT23390.20290574208502782No Hit
GGCCAAAAGTGTAACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTG23210.20134426138492928No Hit
GTTCAGTGGCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG22480.19501158965675183No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGA22060.19136813468985525No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21750.1886789179285744No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG21660.18789817757852512No Hit
GCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCT21450.18607645009507684No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21370.1853824586728108No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA21110.18312698655044624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20720.1797437783668994No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20600.17870279123350036No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20460.17748830624453482No Hit
CTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCT20450.17740155731675158No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCTCTCCCTGCCCGTCACCC20220.17540633197773675No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20170.1749725873388205No Hit
GGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA20100.17436534484433772No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCT19850.1721966216497564No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19810.1718496259386234No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19750.17132913237192388No Hit
CTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT19400.16829291989951004No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19280.16725193276611103No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19250.16699168598276126No Hit
ATCCAGTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGTTGTGGAGA19240.166904937054978No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19180.16638444348827847No Hit
GAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAA19060.16534345635487946No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18970.1645627160048302No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCTCTCC18600.16135300567684982No Hit
CCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG18400.15961802712118478No Hit
GCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC18090.15692881035990397No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18090.15692881035990397No Hit
CCCCTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG17910.1553673296598054No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17750.15397934681527337No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17630.15293835968187433No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGA16950.14703943259261318No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT16910.14669243688148015No Hit
GTGTAAAATCTGTGCCTGATCCAGTGCCACTGAACCTGTCAGGGACCCCG16830.14599844545921412No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16640.14435021583133234No Hit
AGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA16550.14356947548128304No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16480.14296223298680028No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16340.14174774799783477No Hit
GATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG16110.13975252265881993No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGG16100.13966577373103667No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15970.1385380376698544No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15720.1363693144752731No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15510.13454758699182479No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15400.133593348786209No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15350.13315960414729272No Hit
GTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT15240.13220536594167695No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15140.13133787666384444No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC15090.13090413202492818No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15070.13073063416936165No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14980.1299498938193124No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14950.12968964703596264No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14820.12856191097478034No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14820.12856191097478034No Hit
CAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCAGTGCCA14650.12708717920246504No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT14640.1270004302746818No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14510.12587269421349953No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAAT14510.12587269421349953No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14430.1251787027912335No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTC14070.12205574139103641No Hit
CTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCACTGGATCAGGCACAGATTTTACA14050.12188224353546989No Hit
GTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCAGTGCCA13970.12118825211320389No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13650.1184122864241398No Hit
GAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATG13520.11728455036295751No Hit
GTGTAACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATC13370.11598331644620873No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13300.11537607395172594No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTA13260.11502907824059296No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAACG13110.11372784432384417No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13090.11355434646827765No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATCACTATGC12970.11251335933487863No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12960.11242661040709537No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGGGGAGA12950.11233986147931212No Hit
GCTTGGTCCCCTGGCCAAAAGTGTAACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAG12610.10939039793468153No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12530.10869640651241551No Hit
CTCTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12410.10765541937901646No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAT12350.10713492581231696No Hit
GTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCC12250.10626743653448445No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12240.10618068760670119No Hit
CCCATATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCA11920.1034047219176371No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATCACTATGCAGGAGGCTCTGA11830.10262398156758781No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11750.10192999014532181No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11690.10140949657862228No Hit
GTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAAAC11680.10132274765083903No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC11620.10080225408413952No Hit
CCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGA11550.10019501158965674No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCAGTGCC11530.10002151373409024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4650.050.9644432
GTGGTAT5150.046.6833461
GGTATCA16350.045.7941441
GTATCAA44300.041.013151
TATCAAC50000.036.1315732
ATCAACG53050.033.989523
TCAACGC53700.033.5141454
CAACGCA54850.033.061955
AACGCAG55750.032.5282146
CTTATAC2200.031.2232781
CGGCAAT450.004577666530.72807939
ACGCAGA60400.030.0148587
CGCAGAG60650.029.9477468
TATACAC2300.029.8657443
AGAGTAC62150.029.33544511
CAGAGTA62700.029.07811710
ACGAGAC2350.027.77119622
AAATCGT500.007807804727.54700333
GACTTAC500.00790607427.4764861
CGAGACA2400.027.1938123
Produced by FastQC (version 0.11.5)