FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152752
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59860.51927908171055No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40230.3489909364720252No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA38960.33797382264355214No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38690.33563160159340427No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36700.318368564964537No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG35220.30552972365261566No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32420.28124002387330493No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32080.27829056032867433No Hit
CTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT31810.2759483392785265No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC31610.27421336072286145No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31560.27377961608394524No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG30720.26649270615015197No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT30580.26527822116118643No Hit
GTCCCTGACAGGTTCAGTGGCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGAT29000.25157189057143253No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC28420.2465404527600039No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28150.24419823170985605No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26870.23309436895359972No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26750.23205338182020072No Hit
GTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTT26540.2302316543367524No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG25750.22337848904187543No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCACT24730.21453009840798368No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23860.20698294169084072No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23190.20117076352936278No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCC22870.19839479784029868No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG22560.19570558107901787No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAAACT22420.19449109609005233No Hit
CTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT22360.19397060252335283No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22200.1925826196788208No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22060.19136813468985525No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22060.19136813468985525No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21540.18685719044512608No Hit
GGCCAAAAGTGTAACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTG20970.1819125015614807No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20700.1795702805113329No Hit
GTTCAGTGGCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG20510.1779220508834511No Hit
GCACTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCT20340.1764473191111358No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGA19840.17210987272197317No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19560.1696809027440421No Hit
CTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT19530.16942065596069233No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19340.16777242633281053No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCTCTCCCTGCCCGTCACCC19280.16725193276611103No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19250.16699168598276126No Hit
CTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCT19230.16681818812719476No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCT19220.1667314391994115No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18910.16404222243813066No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18670.16196024817133262No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTCCCCTCTCC18610.16143975460463308No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18500.16048551639901731No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA18120.1571890571432537No Hit
GAGTGGAGGCTGAGGATCTTGGAATTTATTACTGCATGCAAGCTCTACAA17550.1522443682596083No Hit
CCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG17540.15215761933182506No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17520.15198412147625856No Hit
GGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA17500.15181062362069206No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17470.1515503768373423No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17170.14894790900384472No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT17110.1484274154371452No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16760.14539120296473135No Hit
GCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC16050.13923202909212043No Hit
ATCCAGTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGTTGTGGAGA15790.13697655696975586No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15780.13688980804197262No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15720.1363693144752731No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCAC15540.13480783377517452No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15510.13454758699182479No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15480.13428734020847502No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGATGACTCAGTCTC15330.13298610629172625No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGA15180.13168487237497745No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15160.13151137451941095No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15120.13116437880827794No Hit
AGTCAGAGCCTCCTGCATAGTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA15100.1309908809527114No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14940.12960289810817938No Hit
GAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATG14840.12873540883034687No Hit
GTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT14810.1284751620469971No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14750.1279546684802976No Hit
GTGTAAAATCTGTGCCTGATCCAGTGCCACTGAACCTGTCAGGGACCCCG14570.12639318778019903No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA14560.1263064388524158No Hit
GATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG14350.12448471136896748No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT14330.12431121351340096No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14210.12327022638000194No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13550.11754479714630728No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT13550.11754479714630728No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATATGGGTTCTAAT13410.11633031215734174No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13210.11459533360167669No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG13070.11338084861271115No Hit
CCCCTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG13040.11312060182936139No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12920.11207961469596237No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12920.11207961469596237No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12730.11043138506808055No Hit
CAGTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCAGTGCCA12460.10808916401793274No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12440.10791566616236623No Hit
CTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCACTGGATCAGGCACAGATTTTACA12390.10748192152344999No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12310.10678793010118395No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12210.10592044082335143No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGGGGAGA11980.1039252154843366No Hit
GTGTAACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATC11980.1039252154843366No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11980.1039252154843366No Hit
GTAATAAATTCCAAGATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCAGTGCCA11940.10357821977320361No Hit
CTCTACAAACTCCTCGTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11930.10349147084542036No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11760.10201673907310506No Hit
GCTTGGTCCCCTGGCCAAAAGTGTAACGAGGAGTTTGTAGAGCTTGCATGCAGTAATAAATTCCAAGATCCTCAG11670.10123599872305579No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAACG11640.10097575193970604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4850.050.290621
TGGTATC4950.048.5510752
GGTATCA17350.046.1346051
GTATCAA49150.040.958541
TATCAAC53900.037.3262942
ATCAACG57000.035.2747963
TCAACGC57450.035.0262574
CAACGCA59000.034.3146555
AACGCAG60100.033.8575976
CGCAGAG64000.031.6873478
ACGCAGA64200.031.5886347
AGAGTAC65450.030.82831611
CAGAGTA66350.030.51340910
AGAGCAT6300.027.77884770
GAGTACG47850.026.34571812
AGTACGG47600.026.26932713
AGTACTT29600.025.11487813
GAGTACT30000.025.00731312
ACGGGAC3050.024.71825216
ACGGGAT2700.024.1148616
Produced by FastQC (version 0.11.5)