FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937945
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.1870045684981529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.10171172083650962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.042.55571
GTGGTAT4450.041.611241
GTATCAA46950.040.0241281
TATCAAC52650.035.9515532
ATCAACG56650.033.4130483
TCAACGC57050.033.298994
CAACGCA58650.032.390585
CTCGTAT1650.031.44001839
AACGCAG60850.031.2758666
CGCGTAG2400.030.03804628
ACGCGTA2450.029.41560227
TGGTATC6500.029.0152472
GACGCGT2500.028.81805826
ACGCAGA66250.028.6108597
CGCAGAG66350.028.5160798
CGTCAGA1603.6379788E-1227.861235
AGAGTAC68800.027.74970811
CAGAGTA69350.027.52963310
GCAGAGT69850.027.234439
GCGTAGT2650.027.21880129
Produced by FastQC (version 0.11.5)