FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076554
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.16720015902592902No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12020.11165255063842594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.042.3437651
GTATCAA43050.039.3330231
TGGTATC7250.038.4514242
GTGGTAT7600.038.0391731
TATCAAC49300.034.3465842
ATCAACG51450.032.7775233
TCAACGC51750.032.5875054
CAACGCA53050.031.9186925
AACGCAG53850.031.4445066
CTCGTAT1750.029.64991839
TCGTATG1900.029.14062940
CGTATGC2050.028.71116641
ACGCAGA59400.028.497247
CGCAGAG59800.028.249098
CAGAGTA62750.026.92104510
GCAGAGT63150.026.6960439
AGAGTAC64300.026.32560211
AGTACGG35450.025.03961213
GAGTACG36050.024.8137412
CGGGATT1251.295557E-724.77750417
Produced by FastQC (version 0.11.5)