FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943461
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40310.20741347523824763No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19990.1028577367901903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11300.044.116961
GTATCAA80600.040.8218731
TATCAAC91400.035.9421232
ATCAACG98000.033.6033443
TCAACGC98750.033.253744
CAACGCA99450.033.0759125
AACGCAG101050.032.584466
GGTATCA42750.031.1236741
CGCAGAG111250.029.7510938
AGAGTAC111850.029.4688411
ACGCAGA112600.029.39447
CAGAGTA114600.028.8215510
GCAGAGT115550.028.6142739
AGTACGG77500.026.15675213
GAGTACG78100.026.042312
GAGTACT54100.023.2038212
AGTACTT55150.022.63882413
TGGTATC21650.022.538372
TACGGGG35000.022.35052315
GTACGGG90550.022.27743114
Produced by FastQC (version 0.11.5)