FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595301
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65340.40957787903348647No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA45250.28364553147023663No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40300.25261690427073014No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTCCCCTAGAGCCTGAAGATG38290.24001740110486985No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCCTCATCGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCCGGCT37320.23393704385567365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35770.22422100907603018No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34810.2182033359221865No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34740.2177645472547187No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33450.20967829895424125No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAG30590.19175064768341524No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTCTTGGCCAGTTGTTACGCTG30170.1891179156786086No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29820.18692397234126978No Hit
GTCCCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTAAC27850.17457520555681968No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27240.17075147574031482No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25580.16034591591179345No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24620.15432824275794976No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT24400.1529491926601939No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24040.15069256522750252No Hit
GCGTAACAACTGGCCAAGACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT22870.14335852607125552No Hit
GACTTCACTCTCACCATCAGTCCCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTAACAACTGG22620.14179142368744208No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCCTCATCGCCTGGTA22490.14097653044785907No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22380.14028700539898115No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21550.13508422548472043No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21350.13383054357766969No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21020.13176196843103588No Hit
CTAGGGGACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCC20840.13063365471469018No Hit
GTTGTTACGCTGCTGACAGTAATAAACTGCAACATCTTCAGGCTCTAGGGGACTGATGGTGAGAGTGAAGT20810.1304456024286326No Hit
ATCTATGATACATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT20600.12912923642622928No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20600.12912923642622928No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20430.12806360680523612No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT20260.12699797718424297No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19840.12436524517943637No Hit
GTTTATTACTGTCAGCAGCGTAACAACTGGCCAAGACTCACTTTCGGCGG19480.12210861774674497No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC19420.12173251317462974No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18560.11634168097431144No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18100.11345821258809466No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17970.11264331934851167No Hit
GTAATAAACTGCAACATCTTCAGGCTCTAGGGGACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17570.11013595553441011No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17210.10787932810171874No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC17170.10762859172030859No Hit
ATCATAGATGATGAGCCGGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCGATGAGGCTGCGAACACTCTGA17140.10744053943425097No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT17030.10675101438537304No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16930.10612417343184766No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16900.10593612114579004No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16850.10562270066902735No Hit
CCCCTAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTAACAA16770.10512122790620705No Hit
GTACCAACAGAAACCTGGCCAGGCTCCCCGGCTCATCATCTATGATACATCCAACAGGGCCACTGGCATCCCAGA16680.10455707104803419No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16620.10418096647591896No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16600.1040555982852139No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA16410.10286460047351566No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16300.10217507542463772No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15960.10004381618265142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.042.224211
GTATCAA63000.041.9541741
TATCAAC72450.036.2733962
GTGGTAT10900.035.0927051
ATCAACG76550.034.2158933
TCAACGC77300.033.9060944
TGGTATC11350.033.3809662
CAACGCA79000.033.1981355
AACGCAG80100.032.869946
CGCAGAG86450.030.3760328
AGAGTAC86700.030.16950811
ACGCAGA87650.029.9993727
GCAGAGT89750.029.3357339
CAGAGTA91300.028.80005610
ACTCGAA950.006665495728.45328170
AGTACGG61250.027.10464113
GAGTACG62050.026.86513112
TCTGGAG10950.025.91976770
GCGTTGT7000.025.05070362
GAGTACT38350.024.64656412
Produced by FastQC (version 0.11.5)