FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578910
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28400.4905771190685944No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19320.33373063170441003No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18500.3195660810834154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18080.31231106735071085No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17120.2957281788188147No Hit
GGGTAACCGTTTCCCCCCCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT16780.2898550724637681No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16750.28933685719714636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16730.28899138035273186No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA14580.2518526195781728No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14240.24597951322312622No Hit
GGATATTAGTAGCTGGTTAGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC14220.24563403637871173No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13600.2349242542018621No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12210.21091361351505417No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11500.1986491855383393No Hit
CGGTTACCCTGTTGACAATGGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC10800.18655749598383167No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTGCAGATGCGACGT10640.18379368122851567No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG10590.1829299891174794No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAA10500.18137534331761415No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10340.17861152856229812No Hit
GTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTA10240.1768841443402256No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10210.17636592907360385No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9760.1685927000742775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.1665198390077905No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9320.16099220949715845No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9230.1594375636972932No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC9020.1558100568309409No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8900.15373719576445388No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA8880.1533917189200394No Hit
GTAGGAGACAGAGTCATCATCACTTGTCGGGCGAATCAGGATATTAGTAGCTGGTTAGCCTGGTATCAGCAGAGA8700.15028242732030886No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8660.14959147363147984No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACCATTGTCAACAGGGTAACCGTTTC8620.14890051994265083No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8470.14630944360954207No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8380.14475479780967682No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGC8310.14354562885422606No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCAT8190.141472767787739No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8070.139399906721252No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8060.13922716829904477No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8030.138708953032423No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7730.13352680036620546No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7730.13352680036620546No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7720.1333540619439982No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACCATTGTCAACAGGGTAACCGTTTCCCCCCCACTTT7680.1326631082551692No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7670.13249036983296195No Hit
GTCCAGATGACCCAGTCTCCATCTTCCGTGTCTGCATTTGTAGGAGACAGAGTCATCATCACTTGTCGGGCGAAT7640.13197215456634018No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7480.12920833981102417No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7470.12903560138881692No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7450.1286901245444024No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7410.1279991708555734No Hit
GTCTCCATCTTCCGTGTCTGCATTTGTAGGAGACAGAGTCATCATCACTTGTCGGGCGAATCAGGATATTAGTAG7390.1276536940111589No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7230.12488987925584288No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT7170.12385344872259936No Hit
GCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTT7170.12385344872259936No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7130.12316249503377037No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTT7090.12247154134494136No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7030.12143511081169785No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCAT6980.12057141870066158No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6910.11936224974521083No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6850.11832581921196732No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTGCAGATGCGACGTCC6780.11711665025651656No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6730.1162529581454803No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATT6680.11538926603444405No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6640.11469831234561503No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6610.11418009707899326No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6540.11297092812354251No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6380.1102071133682265No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6320.10917068283498299No Hit
GTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCAC6310.10899794441277574No Hit
GTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT6260.10813425230173947No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6160.10640686807966696No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGGGGGGGAAACGGTTACCCTGTTGACAATGGTAAGTTGCAAAATCTT6150.10623412965745972No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6130.10588865281304521No Hit
GTCTGCAGCCTGAAGATTTTGCAACTTACCATTGTCAACAGGGTAACCGTTTCCCCCCCACTTTCGGCGGAGGGA6090.1051976991242162No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6040.10433400701317995No Hit
TCCCTGGTCTCTGCTGATACCAGGCTAACCAGCTACTAATATCCTGATTCGCCCGACAAGTGATGATGACT6000.10364305332435093No Hit
CCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACCATTGTCAACAG5960.10295209963552193No Hit
GGTTAGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATC5960.10295209963552193No Hit
ATTGTCAACAGGGTAACCGTTTCCCCCCCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTG5940.10260662279110742No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC5930.10243388436890018No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC5820.1005337617246204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTCG350.005343625356.10089570
CTACGAA200.007254835751.9336742
GTGGTAT1850.050.246231
CGTATGC707.2759576E-1249.43915641
ATCTCCG1750.045.22474310
CGAGCCC1600.045.16329615
ATGCCGT851.8189894E-1244.83669344
TATGCCG851.8189894E-1244.82110243
TCGTATG851.8189894E-1244.76661740
TCCGAGC1800.043.96850213
ACGAGAC1850.042.7949522
CTCCGAG1900.041.65436612
CCGAGCC1850.040.9201614
TATACAC2050.040.305923
GTATCAA20800.039.8901021
CACGAGA1850.039.066921
GCCCACG2000.037.85114718
CTTATAC2100.037.706831
ACCCTAA1950.037.0858927
CCCACGA1950.037.0570619
Produced by FastQC (version 0.11.5)