FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578910
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28060.48470401271354785No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16740.2891641187749391No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16580.2864003040196231No Hit
GGGTAACCGTTTCCCCCCCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT16010.27655421395380975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15670.2706811075987632No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15200.2625624017550224No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15140.26152597122177884No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15040.2597985869997063No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14060.2428702216233957No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA13900.24010640686807969No Hit
CGGTTACCCTGTTGACAATGGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC13410.23164222417992433No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12400.21419564353699194No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG12380.21385016669257742No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12180.2103953982484324No Hit
GGATATTAGTAGCTGGTTAGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC10870.18776666493928246No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10610.18327546596189392No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10280.1775750980290546No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9960.17204746851842256No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9850.1701473458741428No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9760.1685927000742775No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9750.16841996165207027No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTGCAGATGCGACGT9330.16116494791936573No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9250.1597830405417077No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC9240.15961030211950045No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9110.15736470263080618No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8820.15235528838679588No Hit
GTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTA8780.15166433469796686No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA8770.15149159627575962No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8680.14993695047589436No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8640.14924599678706535No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8530.14734587414278558No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8450.14596396676512757No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8170.14112729094332452No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAA8140.14060907567670278No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7970.1376725224991795No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACCATTGTCAACAGGGTAACCGTTTC7900.13646335354372874No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7870.135945138277107No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7850.13559966143269248No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7840.13542692301048523No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7670.13249036983296195No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7650.13214489298854745No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCACGGTT7640.13197215456634018No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACCATTGTCAACAGGGTAACCGTTTCCCCCCCACTTT7440.12851738612219515No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7430.1283446476999879No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7120.12298975661156311No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCAT7080.1222988029227341No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7060.12195332607831962No Hit
GTTGACAATGGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA6910.11936224974521083No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6740.11642569656768754No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGGGGGGGAAACGGTTACCCTGTTGACAATGGTAAGTTGCAAAATCTT6710.11590748130106579No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6700.11573474287885853No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT6640.11469831234561503No Hit
GTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT6610.11418009707899326No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6580.11366188181237152No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6540.11297092812354251No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGC6450.11141628232367726No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6280.10847972914615397No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6260.10813425230173947No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCG6230.10761603703511773No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6190.10692508334628871No Hit
GTCTGCAGCCTGAAGATTTTGCAACTTACCATTGTCAACAGGGTAACCGTTTCCCCCCCACTTTCGGCGGAGGGA6190.10692508334628871No Hit
ATTGTCAACAGGGTAACCGTTTCCCCCCCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTG6180.10675234492408146No Hit
ACCCTGGTCCCTCCGCCGAAAGTGGGGGGGAAACGGTTACCCTGTTGACAATGGTAAGTTGCAAAATCTTCAGG6070.10485222227980169No Hit
GTAGGAGACAGAGTCATCATCACTTGTCGGGCGAATCAGGATATTAGTAGCTGGTTAGCCTGGTATCAGCAGAGA5980.10329757647993643No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5810.10036102330241314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)