FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695659
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39770.23454008146685154No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30900.18223003563806167No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.1804018378695245No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26920.1587583352549068No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26520.15639937039227816No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25600.1509737512082323No Hit
GGATTAGGGACAGATTTTACTCTCACCATCAGCAGTCTCCAACCTGAGGA24460.14425070134974072No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23470.13841226331473486No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22630.13345843710321476No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACACCTATTTAAATTGGTTTCAACAGAAA21440.12644051663689457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21120.12455334474679165No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20820.12278412109982019No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAC20640.1217225869116373No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG19890.11729952779420863No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18880.11134314151607133No Hit
ACTTACTACTGTCAACAAACTTACAGCACCCCGTTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA18420.10863033192404842No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA17800.10497393638697404No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGGAGACTGCTGATGGTGAGAGTAAAATCTGTCCCTAATCCTCTGCCA17530.10338163510469972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17420.10273291976747682No Hit
GTTCAGTGGCAGAGGATTAGGGACAGATTTTACTCTCACCATCAGCAGTCTCCAACCTGAGGATTTTGCAACTTA17250.10173035970085967No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17050.10055087726954534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)