FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542275
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70700.2780973734155432No Hit
GTATAATAGTTATTCTCCCACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT68450.2692470326774248No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGACTGCTGATGCTGAGAGTGAATTCTTTC63560.25001229213991405No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC52720.2073733172060458No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50590.198994994640627No Hit
GTGTATTACTGTGCGAGAGCCGTTAGGGATTACATTTGGGGGAGTTATCG49830.19600554621352922No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACAGTGAATTCTTGGCGTTGTCTCTGGA49790.19584820682262932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49320.1939994689795557No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG48540.190931350857008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45220.1778721814123177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45050.1772034890009932No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42240.1661503967902764No Hit
GAGTATTGGTACCTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCCAAGCTCCTGATCTATAAGGCGT40250.15832276209300725No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37800.1486857244003894No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA37490.1474663441209153No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG37460.1473483395777404No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG37430.1472303350345655No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTACCTGGTTGGCCTGGTATCAGCAGAGA36010.14164478665761965No Hit
GTATTGGTACCTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCC35130.13818332005782222No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33700.1325584368331514No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG33650.13236176259452656No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33390.13133905655367734No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32480.12775958541070498No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32400.1274449066289052No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT32110.12630419604488108No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC31130.12244938096783392No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT31080.12225270672920907No Hit
GGTATCAGCAGAGACCAGGAAAAGCCCCCAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT30410.11961727193163603No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30210.11883057497713663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29950.11780786893628738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29430.11576245685458891No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29210.11489709020463955No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28350.11151429330029206No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27710.10899686304589394No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26870.1056927358369964No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26510.10427668131889743No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGAAAGAATTCA26310.10348998436439803No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC25640.10085454956682499No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25640.10085454956682499No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25470.10018585715550049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)