FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542275
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGACTGCTGATGCTGAGAGTGAATTCTTTC69510.27341652653627163No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68830.27074175689097363No Hit
GTATAATAGTTATTCTCCCACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT63770.25083832394213845No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC47590.18719454032313576No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46750.18389041311423823No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACAGTGAATTCTTGGCGTTGTCTCTGGA46520.1829857116165639No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45050.1772034890009932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44260.17409603603072052No Hit
GTGTATTACTGTGCGAGAGCCGTTAGGGATTACATTTGGGGGAGTTATCG42480.16709443313567574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41280.16237425140867923No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40910.16091886204285533No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37940.149236412268539No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37690.14825304107541473No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG37420.14719100018684053No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36340.1429428366325437No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35390.13920602609867147No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34660.1363345822147486No Hit
GAGTATTGGTACCTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCCAAGCTCCTGATCTATAAGGCGT33940.1335024731785507No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33330.1311030474673275No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA32290.12701222330393053No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31840.12524215515630685No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30960.12178068855650942No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30580.12028596434296053No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG29390.11560511746368902No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTACCTGGTTGGCCTGGTATCAGCAGAGA29000.11407105840241516No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28420.11178963723436687No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28230.1110422751275924No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27990.11009823878219312No Hit
GTATTGGTACCTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCC27890.1097048903049434No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT27140.10675477672557061No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26700.1050240434256719No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26140.10282129195307353No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC26040.10242794347582382No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25710.10112989350089978No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC25580.10061854048047517No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25490.10026452685095043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11750.048.3061641
GTATCAA87750.040.7701871
TATCAAC100000.035.5140342
ATCAACG105100.033.608613
TCAACGC105150.033.466914
CAACGCA106850.033.0473635
AACGCAG108750.032.498986
CGCAGAG116700.030.2556388
AGAGTAC118950.029.82757211
GCAGAGT120850.029.330239
ACGCAGA120750.029.2408547
AGTACGG86800.025.5372813
GAGTACG87350.025.45504612
TGTTAGA17450.024.67793770
GAGTACT53900.024.19115612
AGTACTT54500.023.7359513
GTAATAC17400.023.7240311
GGTATCA75500.023.5103591
GCGTTGT17000.023.38119162
TACGGGC14250.023.1226615
Produced by FastQC (version 0.11.5)