FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090708
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43850.20973756258645398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36870.1763517430458964No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA35560.17008592304616427No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33120.15841523541307537No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG31960.1528668757186561No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC28960.13851766961239925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28770.13760888655900297No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG28410.13588698182625214No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28190.13483470671179335No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26630.1273731195365398No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26040.12455110900230926No Hit
AGCCTGGAGAGTCCCTGACACTCTCCTGTGTAGCCTCTGGATTCACCTTC25990.12431195556720498No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25960.12416846350614241No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24320.11632423083472203No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22380.10704507755267592No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTCCGCCGAAAGTGAGCTCCGGAGGCCAGTAGCTACG22300.10666243205650909No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21760.10407957495738285No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT21410.1024055009116529No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21300.10187936335442346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.044.4278071
GTATCAA74000.040.934761
TATCAAC85000.035.5669252
ATCAACG90050.033.6302763
TCAACGC91000.033.2175064
CAACGCA93000.032.4844975
GTGGTAT11850.032.2804571
AACGCAG94600.032.007746
TGGTATC11950.031.9834862
TCGCTAG450.00470085530.56288315
CGCAGAG102400.029.7374678
ACGCAGA103750.029.2511437
GCAGAGT106100.028.6356539
CAGAGTA107450.028.3398510
AGTACGG73400.025.94269613
AGAGTAC117450.025.86839311
GAGTACG75300.025.46824812
TTCGGGG15450.025.23281970
GTACGGG82500.022.75273114
TACGGGG36300.022.4485615
Produced by FastQC (version 0.11.5)