FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515412
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61260.4042465019413862No Hit
GTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT50460.332978754292562No Hit
CTATCATACTGTAGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC42030.27735031793334086No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41530.27405088517182125No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40620.26804591754585555No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG38290.252670560877174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36290.23947282983109547No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36050.237889102105566No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35420.2337318168260513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32050.2114936400134089No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30410.20067150055562447No Hit
CTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAGCAACTATTTAAATTGGTATCAGCAGAAG25710.16965683259733988No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25630.1691289233554967No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25600.16893095738980554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24540.1619361599353839No Hit
GTCTCCATCCTCCCTGTCTGCATCTCTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAGCAA24400.1610123187621584No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGAGCCACTTCCA24380.16088034145169763No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23460.1548093851705015No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23410.15447944189434953No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21750.14352532512610433No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC21170.13969798312274154No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20550.13560668649845717No Hit
ACATATTACTGTCTACAGTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA20500.13527674322230523No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20470.13507877725661405No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20000.1319773104607856No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19970.1317793444950944No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19770.13045957139048656No Hit
ATATTACTGTCTACAGTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGA19720.13012962811433457No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19470.12847991173357476No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18820.12419064914359923No Hit
GTGCGGTCCCATCAAGCTTCAGCGGCAGTGGATCTGGGACAGAATTCACT18700.12339878528083452No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18450.1217490689000747No Hit
GTCTCTGGTCGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCG18400.12141912562392274No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGAGCCACTTCCA18250.12042929579546685No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18240.12036330714023645No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT18100.11943946596701094No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT18070.11924150000131978No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17650.11646997648164328No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17400.11482026010088346No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16740.11046500885567755No Hit
ATCATACTGTAGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGC16690.11013506557952556No Hit
AGTCAAGACATTAGCAACTATTTAAATTGGTATCAGCAGAAGCCGGGGAA16520.1090132584406089No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16240.1071655760941579No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15760.10399812064309903No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15720.10373416602217746No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT15600.10294230215941276No Hit
GATCTACGATGCCTCCAATTTGGAAACAGGAGTCCCATCAAGGTTCAGTGGAAGTGGCTCTGGGACAGATTTTA15410.1016885177100353No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15330.10116060846819214No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15310.10102863115773136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC150.00238362468.795181
GCTTACG200.0074464951.596391
GTGGTAT5550.046.483231
GTATCAA50750.041.548221
TATCAAC58550.035.7781942
ATCAACG61950.034.09223
TCAACGC62600.033.6832664
CAACGCA63900.033.051835
AACGCAG64550.032.8255846
TATACAC2500.031.6457843
ACGAGAC2400.031.53424822
CGCAGAG70700.029.913638
ACGCAGA71250.029.6827167
AGAGTAC72400.029.3062311
CAGAGTA73650.028.85553410
GCAGAGT74550.028.5071759
CCCACGA2600.027.78083419
AGTATCG500.00785123927.51625818
CTTATAC2550.026.9785021
GAGTACT37200.024.86696212
Produced by FastQC (version 0.11.5)