FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515412
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58210.38411996209611643No Hit
GTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT46800.30882690647823824No Hit
CTATCATACTGTAGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC36770.24264028528215428No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36660.24191441007461997No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG35550.23458966934404635No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35290.23287396430805618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31980.21103171942679616No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31640.2087881051489628No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28690.18932145185599691No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28600.18872755395892338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28210.18615399640493807No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24420.1611442960726192No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23850.1573829427244868No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGAGCCACTTCCA23780.15692102213787407No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23570.1555352603780358No Hit
GTCTCCATCCTCCCTGTCTGCATCTCTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAGCAA23380.15428147592865835No Hit
CTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAGCAACTATTTAAATTGGTATCAGCAGAAG22240.14675876923239356No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21430.14141368815873176No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21270.14035786967504546No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21160.13963199446751115No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20190.13323109491016305No Hit
ATATTACTGTCTACAGTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGA19620.12946974156203067No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19520.12880985500972672No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19420.1281499684574228No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC19410.1280839798021924No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19270.1271601386289669No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19090.12597234283481984No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18620.12287087603899138No Hit
ACATATTACTGTCTACAGTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA18230.12029731848500606No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18070.11924150000131978No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17520.11561212396364816No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT17110.11290658909920206No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17080.11270862313351089No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGAGCCACTTCCA16720.11033303154521674No Hit
GTCTCTGGTCGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCG16680.11006907692429517No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT16670.11000308826906478No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16610.10960715633768242No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16470.10868331516445692No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT16370.10802342861215299No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16270.10736354205984908No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16090.106175746265702No Hit
GTGCGGTCCCATCAAGCTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15900.10492196181632453No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15720.10373416602217746No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15570.10274433619372157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6850.049.2453651
GTATCAA53400.038.675851
TATCAAC60750.033.9151952
ATCAACG65150.031.6544533
TCAACGC65650.031.4889454
CAACGCA67150.030.8725345
AACGCAG68300.030.5517146
ACGCAGA73750.028.3405287
CGCAGAG73700.028.3131878
CATGCGG950.006839042528.30373670
AGAGTAC73750.028.15438311
CAGAGTA74800.027.8050510
GCAGAGT75000.027.6393839
AGTACGG52300.025.72561313
GAGTACG53000.025.4489212
ACGGGGT4450.023.9196616
TACGGGG21800.023.14954815
TACTGAT7500.022.40712470
GTACGGG61150.022.06148514
ATCATTT9200.021.92001370
Produced by FastQC (version 0.11.5)