FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838199
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.10737307011819389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32450.039.6858251
GGTATCA14000.037.6306651
GTGGTAT6900.034.433291
ATCAACG38850.033.502663
TATCAAC38700.033.4545672
TCAACGC39350.033.076964
CAACGCA40000.032.7116245
TGGTATC6650.032.621012
AACGCAG41050.031.874916
ACGCAGA44950.029.1789827
CGCAGAG46650.027.894278
CGCAACG500.00781395227.541715
AGAGTAC47500.027.39511111
CAGAGTA48300.026.87008710
GCAGAGT49500.026.2882379
AGTACGG27500.025.16159813
GAGTACG27650.025.14960112
ACGGATC700.00135054624.5893388
CGAATTA1151.7282364E-623.94930615
TACGGGA10900.022.42500915
Produced by FastQC (version 0.11.5)