FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194415
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46520.21199271787697405No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44910.2046559105729773No Hit
GAGTTACAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33540.15284255712798173No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32580.14846781488460478No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29550.13466003467894633No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT29430.1341131918985242No Hit
ACAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA28930.13183468031343204No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28800.13124226730130809No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27140.12367760883880215No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAAAAA26700.12167251864392105No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA26680.12158137818051735No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA26330.11998642007095285No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25440.11593066944948882No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25220.11492812435204827No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT25190.11479141365694273No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23760.10827487052357918No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23480.10699890403592757No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG23340.10636092079210177No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC22770.10376341758509672No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22640.10317100457297275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9950.049.79691
GTATCAA82950.040.4022451
TATCAAC89550.037.05672
ATCAACG97950.033.779273
TCAACGC98300.033.5645944
CAACGCA100650.032.768225
AACGCAG101800.032.5995836
ACGCAGA108400.030.5514187
ATAAACG802.853396E-630.0632883
CGCAGAG110550.029.9262038
AGAGTAC112050.029.52491211
GCAGAGT114950.028.8105569
CAGAGTA117150.028.2402210
AGTACGG78550.025.4733113
GAGTACG80000.025.26671412
GTAATAC11650.024.80941
GGTATCA54300.024.5230261
GAGTACT53250.024.3610312
AGTACTT53000.024.21914313
ACGGGTA1803.6561687E-1022.88813216
Produced by FastQC (version 0.11.5)