FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573830
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.17555898667581632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.045.53121
GTATCAA66250.040.267691
GTGGTAT8350.034.995591
TATCAAC78250.034.3549652
TCAACGC81100.033.062894
ATCAACG80800.033.0580143
CAACGCA82800.032.3840645
TGGTATC8900.031.6731262
AACGCAG85750.031.3501576
ACGCAGA91550.029.2833357
CGCAGAG92000.029.1401028
AGAGTAC94950.028.23474911
CAGAGTA97300.027.62346810
GCAGAGT100200.026.7553849
GAGTACG61600.024.60606812
AGTACGG61700.024.56618713
ACGGGTA2450.023.85110716
ACCGATC3350.023.62759827
GTATCGG1052.270386E-523.01448835
GTACGGG69000.022.01836614
Produced by FastQC (version 0.11.5)