FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573830
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34130.2168595083331745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16850.10706365998869001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.044.1850661
GTATCAA72850.042.9753951
GTGGTAT8850.039.6084631
TATCAAC83450.037.450342
TCAACGC87650.035.573594
ATCAACG88250.035.4319953
TGGTATC9350.035.2614632
CAACGCA90100.034.700635
AACGCAG91300.034.3936426
ACGCAGA98550.031.8982037
CGCAGAG100550.031.2978218
AGAGTAC101900.030.84953711
CAGAGTA103650.030.36175510
GCAGAGT106250.029.586529
GAGTACG63150.027.46735412
AGTACGG63000.027.31684113
GAGTACT58150.024.22879412
TAACGGC2450.023.94888736
GTACGGG73600.023.4795114
AGTACTT59050.023.4546413
Produced by FastQC (version 0.11.5)