FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969509
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60600.6250586637153446No Hit
GATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA50910.5251111645172969No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36980.38143018785797755No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36830.3798830129477911No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36810.37967672295976623No Hit
GTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT36360.3750351982292067No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34180.35254958953449633No Hit
AGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC33680.3473923398338747No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33040.34079106021707894No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31450.3243910061691021No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG31380.32366899121101506No Hit
CTTTCATACTGTTGGCAGTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC31370.32356584621700263No Hit
CTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC30130.3107758669594609No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28210.29097202810907374No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTA27560.28426760349826563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27330.2818952686359797No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25630.26436061965386604No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25110.25899707996521953No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA25030.25817192001312006No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA24780.2555932951628092No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG23360.24094670601304374No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC23210.23939953110285722No Hit
CTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT22390.2309416415938377No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22000.22691898682735281No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21940.2263001168632782No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21920.22609382687525334No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21650.22330891203691766No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC21080.21742964737820897No Hit
ACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA20700.21351013760573653No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20570.21216925268357487No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20390.2103126427913511No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20320.20959062783326407No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19980.20608369803684132No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19870.20494910310270456No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19840.20463966812066725No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19610.20226733325838128No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19290.19896669344998344No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19210.19814153349788396No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18700.1928811388032499No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17610.1816383344558947No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17410.17957543457564604No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17310.1785439846355217No Hit
GCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAG17180.17720309971336007No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17150.17689366473132276No Hit
GTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG16990.17524334482712384No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16880.17410874989298705No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16550.17070496509057678No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16520.17039553010853947No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16200.16709489030014163No Hit
ATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15630.16121562564143294No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15610.16100933565340805No Hit
CACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAAC14870.153376606096488No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAG14830.15296402612043827No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA14730.15193257618031394No Hit
GCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGA14230.1467753264796923No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14060.14502186158148095No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCAT14010.14450613661141876No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13760.14192751176110796No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13730.14161807677907065No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13450.13873001694672252No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13400.13821429197666035No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13270.13687340705449874No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13190.13604824710239924No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13120.13532623214431222No Hit
GTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGG13000.13408849221616304No Hit
GGCCAAAATTGTACGAGTAACTTTCATACTGTTGGCAGTAATAAGTTGCC12780.1318193023478895No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12510.12903438750955382No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGG12410.12800293756942946No Hit
TCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT12130.12511487773708135No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC12000.12377399281491971No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11990.12367084782090729No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11950.12325826784485756No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11880.12253625288677053No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11830.12202052791670835No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAGT11740.12109222297059646No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACA11740.12109222297059646No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11710.12078278798855915No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11620.11985448304244727No Hit
CAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11580.11944190306639751No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11570.11933875807238509No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11560.11923561307837266No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11460.11820416313824834No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11350.11706956820411157No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11310.11665698822806182No Hit
CACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC11220.11572868328194993No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT11060.11407836337775101No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11010.11356263840768883No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC10420.1074770837609553No Hit
ACCTTGATGGGACCCCAGTCTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG10390.10716764877891799No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGC10380.10706450378490555No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCAGTCAGGACACAGCATGGA10230.10551732887471907No Hit
GCTTGGTCCCCTGGCCAAAATTGTACGAGTAACTTTCATACTGTTGGCAGTAATAAGTTGCCATGTCATCAGG10180.10500160390465689No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTGTACGA10060.10376386397650769No Hit
ATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA9930.10242297905434608No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9860.10170096409625905No Hit
TTCATACTGTTGGCAGTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGC9840.10149467410823416No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAGTCTCTAAGCTCGA9840.10149467410823416No Hit
CTCCAGCTTGGTCCCCTGGCCAAAATTGTACGAGTAACTTTCATACTGTT9820.1012883841202093No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9770.10077265915014713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)