FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400734
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.10131408864733214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA200.006848408452.6932459
GTACCGT200.007458775351.567916
GTATCAA18350.040.8421551
GGTATCA7150.038.9463921
GTGGTAT2850.036.1880071
TATCAAC21350.035.1032142
ACGACTC400.00265089134.348588
ATCAACG21900.034.2216263
TCAACGC22200.033.759174
CAACGCA22900.032.727235
TGGTATC3200.032.2299422
AACGCAG23400.032.027936
CGTATGC1001.3693352E-831.22149341
CTCGTAT1001.3966201E-831.15866739
TCGTATG1052.2206223E-829.70854840
ACGCAGA25500.029.6340687
CGCAGAG25750.029.2129658
AGTCGGA605.5571826E-428.62381421
AGAGTAC26050.028.61282511
TCTCGTA1103.5808625E-828.28337338
Produced by FastQC (version 0.11.5)