FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996670
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.12973200758525893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37200.041.2306671
GCGAATT650.00160152438.0653270
GGTATCA15500.036.60841
TATCAAC42800.035.5163882
GTGGTAT5700.034.9930341
ATCAACG45100.033.7813843
TCAACGC45400.033.633914
CAACGCA46650.032.8064045
AACGCAG47650.032.2622686
ACGCAGA52500.029.3341057
CGCAGAG53450.028.8127298
AGAGTAC55000.028.06182711
GCAGAGT56250.027.3771259
CAGAGTA56500.027.25598710
TGGTATC7050.026.8301962
CTTATAC4050.025.4738561
TATACAC4350.025.2994443
ATCTCCG3800.024.4237610
CGGGCTA1701.7462298E-1024.26534317
ACGAGAC3850.024.10777722
Produced by FastQC (version 0.11.5)