FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996670
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29420.29518295925431687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.042.2367321
GTATCAA44700.038.0031051
TAAGCGC501.918174E-434.32354423
TATCAAC49900.033.4108852
GTGGTAT7150.033.1850661
ATCAACG53050.031.201243
TCAACGC53150.031.0482924
TGGTATC7550.030.9605542
CAACGCA54950.030.0831095
AACGCAG55800.029.563396
TAAGCGT1105.3855125E-428.77996370
ACGCAGA60150.027.5394257
CGCAGAG60250.027.4367948
AGAGTAC62100.026.56420511
GCAGAGT63200.026.2103869
CAGAGTA63050.026.16395410
GTGCTAC1101.1858119E-625.0090351
GAGTACG37450.024.08503312
AGTACGG37500.024.05412913
GAGTACT34600.021.70757712
Produced by FastQC (version 0.11.5)