FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544100
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.11229553390920788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24500.042.5594521
GTGGTAT3650.038.655431
TATCAAC28950.035.898632
GGTATCA9450.035.6873051
ATCAACG29300.035.4698073
TCAACGC29450.035.4064
CAACGCA30000.034.7568865
TGGTATC4300.033.6124652
AACGCAG31200.033.3097886
ACGCAGA33750.030.7817297
CGCAGAG33950.030.6003938
AGAGTAC35100.029.59781511
CAGAGTA35750.029.155910
GACGCGG605.499363E-428.67730122
GCAGAGT36300.028.6193759
GCAGCGT1800.026.765481
GAGTACG20850.026.56315412
ATCTCCG1306.488335E-926.46162210
AGCGTCA1705.456968E-1226.3156413
AGTACGG21300.026.16346413
Produced by FastQC (version 0.11.5)