FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839929
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27520.3276467415698232No Hit
CTATGATAGTATCCCGTATTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT26710.3180030693070486No Hit
CTATCATAGTGTTGACAGTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATATGT24800.29526305199606157No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG24770.29490587894929216No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19470.23180530735335964No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19430.23132907662433375No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17520.20858905931334673No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17000.20239805983600997No Hit
ATAATACACAGCCGTGTCCTCAGTTCTCAGGTTGTTCATTTGCAGATACA16620.19787386791026385No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16270.19370684903128715No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGAA15450.1839441190862561No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14910.1775150042444064No Hit
ATATTACTGTCAACACTATGATAGTATCCCGTATTCTTTTGGCCAGGGGA14610.1739432737767121No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14470.1722764662251214No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC14430.17180023549609552No Hit
CAATAATACACAGCCGTGTCCTCAGTTCTCAGGTTGTTCATTTGCAGATA14330.17060965867353073No Hit
GTAGGAGACAGAGTCTCCATCACTTGCCAGGCGAGTCAGGACATTAGCAAATATTTAAATTGGTATCAACAGAGA14310.17037154330901777No Hit
GACCAGGGAAGGCCCCTAAACTCCTGATCTACGATGCATCCAATTTGGAA14230.16941908185096596No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13960.1662045244300411No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCTCCATCACTT13850.16489488992521986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13420.1597754095881914No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCACTGCATGCCATAGTCAC13320.15858483276562663No Hit
AGTCAGGACATTAGCAAATATTTAAATTGGTATCAACAGAGACCAGGGAA13250.15775142898983127No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13020.15501310229793233No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12970.15441781388664994No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12690.1510841987834686No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12520.1490602181851085No Hit
CAGTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATATGTCTCAGATCCACTTCCA12500.14882210282059555No Hit
GTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATATGTCTCAGATCCACTTCCA12410.14775058368028726No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12290.14632189149320954No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT12220.1454884877174142No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTGACTATGGCATGCAGTGGGTCCGCCAGGCTCCAGGCAAGGGG11820.14072618042715515No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11770.14013089201587275No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11770.14013089201587275No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT11720.13953560360459039No Hit
TCCCTGGTCTCTGTTGATACCAATTTAAATATTTGCTAATGTCCTGACTCGCCTGGCAAGTGATGGAGACT11670.13894031519330802No Hit
ATATATTACTGTCAACACTATGATAGTATCCCGTATTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11570.13774973837074325No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11090.13203496962243239No Hit
ATCATAGTGTTGACAGTAATATATTGCAATATCTTCAGGCTGCAGGCTGC10990.1308443927998676No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10970.13060627743535466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10940.13024910438858522No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10940.13024910438858522No Hit
GTCTGCATCTGTAGGAGACAGAGTCTCCATCACTTGCCAGGCGAGTCAGG10590.12608208550960856No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10590.12608208550960856No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10510.12512962405155673No Hit
CTCCATCACTTGCCAGGCGAGTCAGGACATTAGCAAATATTTAAATTGGTATCAACAGAGACCAGGGAAGGCCC10250.12203412431288836No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10110.12036731676129768No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9850.1172718170226293No Hit
ATAGTATCCCGTATTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA9660.11500972105975625No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9640.11477160569524328No Hit
GTATCAACAGAGACCAGGGAAGGCCCCTAAACTCCTGATCTACGATGCAT9610.11441443264847385No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9610.11441443264847385No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9510.11322385582590909No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9420.11215233668560082No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9390.11179516363883138No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9390.11179516363883138No Hit
GTTCTCAGGTTGTTCATTTGCAGATACAGGGTGTTCTTGGAATTGTCTCT9370.11155704827431842No Hit
ACAGAGACCAGGGAAGGCCCCTAAACTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTT9370.11155704827431842No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9250.11012835608724071No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9150.10893777926467595No Hit
GAGTAAAATATGTCTCAGATCCACTTCCACTGAACCTTGATGGGACCCCT9080.1081043754888806No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9050.10774720244211117No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAG8990.1070328563485723No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC8880.10572322184375109No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8850.10536604879698165No Hit
CACTATGATAGTATCCCGTATTCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT8820.10500887575021223No Hit
GGGCTGGAGTGGGTGGCAGTCATTTCTGATGATGGAAGTAACAAATACTA8820.10500887575021223No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8800.10477076038569928No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG8760.10429452965667337No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8550.10179431832928736No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8490.1010799722357485No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8460.10072279918897907No Hit
ATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAATATATTACTGTCAACACTATGATAGTATCC8430.10036562614220963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)