FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064298
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.12233415829025329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44350.039.4762731
GGTATCA19150.038.5415571
GTGGTAT6050.037.4496841
TATCAAC52700.033.4137732
ATCAACG54600.032.2510223
TCAACGC54900.032.137314
TGGTATC6900.031.8382852
CAACGCA56350.031.3712675
AACGCAG58450.030.3028856
ACGCAGA62500.028.3312667
CGCAGAG62850.028.1734988
AGAGTAC65650.026.97188611
CAGAGTA67250.026.53428610
GAGTACG39950.026.37047212
GCAGAGT67600.026.2953769
AGTACGG41050.025.74742913
TACGGGG17500.024.12036115
GTACGGG44950.023.4371614
AGCGTCA2005.638867E-1122.3116723
TACGGGA13850.021.80467415
Produced by FastQC (version 0.11.5)